Specificity
CSI 001-74 is highly specific for perlecan. There is no evidence for cross-reactivity with other connectivetissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen
Bovine corneal endothelial cells
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Epitope Specificity
Epitope is located in domain V
Unit Size
200 µL: Cat. No. CSI 001-74-021 mL: Cat. No. CSI 001-74-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.
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